The Princess [link]: Adorable webcomic about a gradeschool-aged transgirl. Includes multiple trans characters (including a nonbinary best friend person!), and the protagonist is bisexual. Just frickin adorably amazingly awesomely cute. TW for nonexplicit depictions of abuse.
Menage a 3 (ma3) [link]: TW for transmisogyny, misogyny, rape culture, and nonconsent passed off as wacky antics. Gross. Hugely gross. Like, I-can’t-believe-this-was-premeditated gross. Includes the phrase “is she really a man” within the first 40 pages. Also includes canonically bisexual and gay characters who still act grossly and apparently there’s a translady later in the comic. I don’t know if it’s still running. Cast seems to be all white.
Curvy [link]: TW for lots and lots of explicit sex. Like, a lot. Definitely NSFW. A cute fantasty comic with some unique world-building where everybody seems to be canonically bisexual. Gender isn’t really discussed although there is one trans-man side character who’s gender is never invalidated ever so that was cool. Includes characters of color, one of the main protagonists is black. Still running, I would definitely recommend this one.
Girls With Slingshots [link]: Cute slice-of-life comic with recurring queer characters. Adorable depiction of an asexual relationship. Cissexist in a trans-people-don’t-seem-to-exist-here way. Unfortunately binarist, has a tendency to break characters off into “boys” and “girls” groups. I still totally follow it though. Still running. Includes characters of color, but cast is overwhelmingly white.
Go Get A Roomie [link]: TW for some serious dudebro-ish antics on the part of the lesbian protagonist and cissexism in the form of trans people not generally existing and equating body parts to gender. You meet one trans character early on, once and never again, and much is made of the fact that he “doesn’t have boobs anymore”. Cue cisfuckery. Also sex happens a lot. It gets better though, mostly. I forgave it it’s problematic stuff for sake of some neat fantasy elements and a cute art style and the fact that it included so many lesbians. Cast is overwhelmingly but not exclusively white and one of the main characters uses a wheelchair. Still running.
Penny and Aggie [link]: Shares an author with Ma3 and is surprisingly tolerable despite this. Lots of wacky teenage girl antics, some hard core Not Like Other Girls syndrome but passes the Benchdel test. Main protagonists and antagonists are girls. Cissexist in a trans-people-don’t-seem-to-exist-here way. Includes canonically queer characters. I don’t know if it’s still running, I noped-out after the only two characters with mental illness turned out to be murderous lunatics. Largely but not exclusively white cast.
Red String [link]: Largely centered around the trails and tribulation of a cishet couple, but one of the main characters is a lesbian and her story-arch does a pretty good job of portraying the whole coming-out-as-a-teenager thing. Some sexual content, rarely explicit. Cast is overwhelmingly Japanese which makes sense because the comic is set in a series of Japanese highschools. Finished running.
Questionable Content [link]: Another cute slice-of-life comic, notable for its multiple recurring queer characters and talking robots. Despite relying largely on butt jokes and slapstic for humor it’s refreshingly free of “lol girls/boys don’t do that isn’t it funny that you’re doing that” punchlines. Also bonus points for portraying OCD accurately. Includes one translady who is handled respectfully and carefully by the author. She’s believable and multidimensional and hella cute. Still running.
A Softer World [link]: TW for depression and suicide. One of my favourite comics. Has some recurring queer themes but is largely a depression comic. I just love it okay. Still running.
YU + ME [link]: TW for depression, suicide, violence (especially later on) and depictions of abuse. A fantasy webcomic that starts out looking like a highschool lesbian drama. Lots of lesbians. Did I mention lesbians. Overwhelmingly lesbians. Cissexist in that trans people don’t exist even in magical fantasy realms which kind of irritates me. The artist does some neat things with swapping out art styles after the highschool part of the comic is concluded. Cast is mostly white, includes some narratively important characters of color. The main antagonist is a Japanese woman with a really neat story arch. Finished running.
DAR [link]: A comic about a college-aged lesbian. Cissexist in a trans-people-don’t-really-exist way. Often funny, often cute. I don’t have a lot to say about this one because I haven’t read it all the way through but it’s often recommended so I thought I’d include it. Author also does some adorable sex-toy reviews and PSA comics.